Device

Part:BBa_K2082002:Design

Designed by: Marius Schöller   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Split CFP Bimolecular fluorescence complementation (BiFC) with anti-GFP-Nanobody


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 215
    Illegal AgeI site found at 467
    Illegal AgeI site found at 590
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E. coli


Source

Genesynthesis

References